Review



excitatory synapses  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Cell Signaling Technology Inc excitatory synapses
    CLR01 reverts the loss of glutamatergic synapses in cortical neurons caused by pathological Atx3. Rat cortical neurons were transfected with plasmids encoding eGFP, wild‐type eGFP‐Atx3 28Q, or mutant eGFP‐Atx3 84Q. a) Neurons were immunolabeled for MAP2, PSD95, and vGLUT1. <t>Excitatory</t> synapses were detected as PSD95 puncta that colocalized with vGLUT1; scale bar = 10 µm. b) Integrated fluorescence intensity of PSD95 puncta colocalized with vGLUT1 ( n = 33–36 neurons per condition in 3 independent experiments). Statistical analyses were performed using Kruskal–Wallis and Dunn's post‐hoc test (Table , Supporting Information). c) Neurons incubated with 10 µ m CLR03 or CLR01 were immunolabeled for MAP2, PSD95, and VGluT1 clusters; scale bar = 10 µm. d) Integrated fluorescence intensity of PSD95 puncta colocalized with VGluT1 ( n = 35–41 neurons per condition in each of 3 independent experiments. Statistical analyses were performed using Kruskal–Wallis and Dunn's post‐hoc tests (Table , Supporting Information). In panels (c) and (d), boxes show the 25th and 75th percentiles, whiskers range from the minimum to the maximum values, and the horizontal line shows the median value. Mean is represented by “+.” e) GFP‐Atx3 accumulates in the cell body of a fraction of transfected cortical cultures treated with CLR03 or CLR01; scale bars = 300, 15 µm. f) Percentage of cells with GFP‐Atx3 aggregates ( n = 3 independent experiments, 31–35 neurons per condition). Statistical analyses were performed using two‐way ANOVA and Sidak's multiple comparisons post‐hoc tests (Table , Supporting Information). p ‐values are indicated in the graphs shown in panels (b), (d), and (f). Data are represented as mean ± SEM.
    Excitatory Synapses, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1202 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/excitatory+synapses/pmc12931234-300-3-9?v=Cell+Signaling+Technology+Inc
    Average 96 stars, based on 1202 article reviews
    excitatory synapses - by Bioz Stars, 2026-08
    96/100 stars

    Images

    1) Product Images from "Allosteric Modulation of Pathological Ataxin‐3 Aggregation: A Path to Spinocerebellar Ataxia Type‐3 Therapies"

    Article Title: Allosteric Modulation of Pathological Ataxin‐3 Aggregation: A Path to Spinocerebellar Ataxia Type‐3 Therapies

    Journal: Advanced Science

    doi: 10.1002/advs.202502216

    CLR01 reverts the loss of glutamatergic synapses in cortical neurons caused by pathological Atx3. Rat cortical neurons were transfected with plasmids encoding eGFP, wild‐type eGFP‐Atx3 28Q, or mutant eGFP‐Atx3 84Q. a) Neurons were immunolabeled for MAP2, PSD95, and vGLUT1. Excitatory synapses were detected as PSD95 puncta that colocalized with vGLUT1; scale bar = 10 µm. b) Integrated fluorescence intensity of PSD95 puncta colocalized with vGLUT1 ( n = 33–36 neurons per condition in 3 independent experiments). Statistical analyses were performed using Kruskal–Wallis and Dunn's post‐hoc test (Table , Supporting Information). c) Neurons incubated with 10 µ m CLR03 or CLR01 were immunolabeled for MAP2, PSD95, and VGluT1 clusters; scale bar = 10 µm. d) Integrated fluorescence intensity of PSD95 puncta colocalized with VGluT1 ( n = 35–41 neurons per condition in each of 3 independent experiments. Statistical analyses were performed using Kruskal–Wallis and Dunn's post‐hoc tests (Table , Supporting Information). In panels (c) and (d), boxes show the 25th and 75th percentiles, whiskers range from the minimum to the maximum values, and the horizontal line shows the median value. Mean is represented by “+.” e) GFP‐Atx3 accumulates in the cell body of a fraction of transfected cortical cultures treated with CLR03 or CLR01; scale bars = 300, 15 µm. f) Percentage of cells with GFP‐Atx3 aggregates ( n = 3 independent experiments, 31–35 neurons per condition). Statistical analyses were performed using two‐way ANOVA and Sidak's multiple comparisons post‐hoc tests (Table , Supporting Information). p ‐values are indicated in the graphs shown in panels (b), (d), and (f). Data are represented as mean ± SEM.
    Figure Legend Snippet: CLR01 reverts the loss of glutamatergic synapses in cortical neurons caused by pathological Atx3. Rat cortical neurons were transfected with plasmids encoding eGFP, wild‐type eGFP‐Atx3 28Q, or mutant eGFP‐Atx3 84Q. a) Neurons were immunolabeled for MAP2, PSD95, and vGLUT1. Excitatory synapses were detected as PSD95 puncta that colocalized with vGLUT1; scale bar = 10 µm. b) Integrated fluorescence intensity of PSD95 puncta colocalized with vGLUT1 ( n = 33–36 neurons per condition in 3 independent experiments). Statistical analyses were performed using Kruskal–Wallis and Dunn's post‐hoc test (Table , Supporting Information). c) Neurons incubated with 10 µ m CLR03 or CLR01 were immunolabeled for MAP2, PSD95, and VGluT1 clusters; scale bar = 10 µm. d) Integrated fluorescence intensity of PSD95 puncta colocalized with VGluT1 ( n = 35–41 neurons per condition in each of 3 independent experiments. Statistical analyses were performed using Kruskal–Wallis and Dunn's post‐hoc tests (Table , Supporting Information). In panels (c) and (d), boxes show the 25th and 75th percentiles, whiskers range from the minimum to the maximum values, and the horizontal line shows the median value. Mean is represented by “+.” e) GFP‐Atx3 accumulates in the cell body of a fraction of transfected cortical cultures treated with CLR03 or CLR01; scale bars = 300, 15 µm. f) Percentage of cells with GFP‐Atx3 aggregates ( n = 3 independent experiments, 31–35 neurons per condition). Statistical analyses were performed using two‐way ANOVA and Sidak's multiple comparisons post‐hoc tests (Table , Supporting Information). p ‐values are indicated in the graphs shown in panels (b), (d), and (f). Data are represented as mean ± SEM.

    Techniques Used: Transfection, Mutagenesis, Immunolabeling, Fluorescence, Incubation



    Similar Products

    excitatory synapses  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc excitatory synapses
    CLR01 reverts the loss of glutamatergic synapses in cortical neurons caused by pathological Atx3. Rat cortical neurons were transfected with plasmids encoding eGFP, wild‐type eGFP‐Atx3 28Q, or mutant eGFP‐Atx3 84Q. a) Neurons were immunolabeled for MAP2, PSD95, and vGLUT1. <t>Excitatory</t> synapses were detected as PSD95 puncta that colocalized with vGLUT1; scale bar = 10 µm. b) Integrated fluorescence intensity of PSD95 puncta colocalized with vGLUT1 ( n = 33–36 neurons per condition in 3 independent experiments). Statistical analyses were performed using Kruskal–Wallis and Dunn's post‐hoc test (Table , Supporting Information). c) Neurons incubated with 10 µ m CLR03 or CLR01 were immunolabeled for MAP2, PSD95, and VGluT1 clusters; scale bar = 10 µm. d) Integrated fluorescence intensity of PSD95 puncta colocalized with VGluT1 ( n = 35–41 neurons per condition in each of 3 independent experiments. Statistical analyses were performed using Kruskal–Wallis and Dunn's post‐hoc tests (Table , Supporting Information). In panels (c) and (d), boxes show the 25th and 75th percentiles, whiskers range from the minimum to the maximum values, and the horizontal line shows the median value. Mean is represented by “+.” e) GFP‐Atx3 accumulates in the cell body of a fraction of transfected cortical cultures treated with CLR03 or CLR01; scale bars = 300, 15 µm. f) Percentage of cells with GFP‐Atx3 aggregates ( n = 3 independent experiments, 31–35 neurons per condition). Statistical analyses were performed using two‐way ANOVA and Sidak's multiple comparisons post‐hoc tests (Table , Supporting Information). p ‐values are indicated in the graphs shown in panels (b), (d), and (f). Data are represented as mean ± SEM.
    Excitatory Synapses, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/excitatory+synapses/pmc12931234-300-3-9?v=Cell+Signaling+Technology+Inc
    Average 96 stars, based on 1 article reviews
    excitatory synapses - by Bioz Stars, 2026-08
    96/100 stars
    		  Buy from Supplier
    excitatory synapses  (Oxford Instruments)
    99
    Oxford Instruments excitatory synapses
    CLR01 reverts the loss of glutamatergic synapses in cortical neurons caused by pathological Atx3. Rat cortical neurons were transfected with plasmids encoding eGFP, wild‐type eGFP‐Atx3 28Q, or mutant eGFP‐Atx3 84Q. a) Neurons were immunolabeled for MAP2, PSD95, and vGLUT1. <t>Excitatory</t> synapses were detected as PSD95 puncta that colocalized with vGLUT1; scale bar = 10 µm. b) Integrated fluorescence intensity of PSD95 puncta colocalized with vGLUT1 ( n = 33–36 neurons per condition in 3 independent experiments). Statistical analyses were performed using Kruskal–Wallis and Dunn's post‐hoc test (Table , Supporting Information). c) Neurons incubated with 10 µ m CLR03 or CLR01 were immunolabeled for MAP2, PSD95, and VGluT1 clusters; scale bar = 10 µm. d) Integrated fluorescence intensity of PSD95 puncta colocalized with VGluT1 ( n = 35–41 neurons per condition in each of 3 independent experiments. Statistical analyses were performed using Kruskal–Wallis and Dunn's post‐hoc tests (Table , Supporting Information). In panels (c) and (d), boxes show the 25th and 75th percentiles, whiskers range from the minimum to the maximum values, and the horizontal line shows the median value. Mean is represented by “+.” e) GFP‐Atx3 accumulates in the cell body of a fraction of transfected cortical cultures treated with CLR03 or CLR01; scale bars = 300, 15 µm. f) Percentage of cells with GFP‐Atx3 aggregates ( n = 3 independent experiments, 31–35 neurons per condition). Statistical analyses were performed using two‐way ANOVA and Sidak's multiple comparisons post‐hoc tests (Table , Supporting Information). p ‐values are indicated in the graphs shown in panels (b), (d), and (f). Data are represented as mean ± SEM.
    Excitatory Synapses, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/excitatory+synapses/pm38832126-130-19-23?v=Oxford+Instruments
    Average 99 stars, based on 1 article reviews
    excitatory synapses - by Bioz Stars, 2026-08
    99/100 stars
    		  Buy from Supplier
    ca1 excitatory synapses  (Oxford Instruments)
    99
    Oxford Instruments ca1 excitatory synapses
    CLR01 reverts the loss of glutamatergic synapses in cortical neurons caused by pathological Atx3. Rat cortical neurons were transfected with plasmids encoding eGFP, wild‐type eGFP‐Atx3 28Q, or mutant eGFP‐Atx3 84Q. a) Neurons were immunolabeled for MAP2, PSD95, and vGLUT1. <t>Excitatory</t> synapses were detected as PSD95 puncta that colocalized with vGLUT1; scale bar = 10 µm. b) Integrated fluorescence intensity of PSD95 puncta colocalized with vGLUT1 ( n = 33–36 neurons per condition in 3 independent experiments). Statistical analyses were performed using Kruskal–Wallis and Dunn's post‐hoc test (Table , Supporting Information). c) Neurons incubated with 10 µ m CLR03 or CLR01 were immunolabeled for MAP2, PSD95, and VGluT1 clusters; scale bar = 10 µm. d) Integrated fluorescence intensity of PSD95 puncta colocalized with VGluT1 ( n = 35–41 neurons per condition in each of 3 independent experiments. Statistical analyses were performed using Kruskal–Wallis and Dunn's post‐hoc tests (Table , Supporting Information). In panels (c) and (d), boxes show the 25th and 75th percentiles, whiskers range from the minimum to the maximum values, and the horizontal line shows the median value. Mean is represented by “+.” e) GFP‐Atx3 accumulates in the cell body of a fraction of transfected cortical cultures treated with CLR03 or CLR01; scale bars = 300, 15 µm. f) Percentage of cells with GFP‐Atx3 aggregates ( n = 3 independent experiments, 31–35 neurons per condition). Statistical analyses were performed using two‐way ANOVA and Sidak's multiple comparisons post‐hoc tests (Table , Supporting Information). p ‐values are indicated in the graphs shown in panels (b), (d), and (f). Data are represented as mean ± SEM.
    Ca1 Excitatory Synapses, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/excitatory+synapses/pm36472212-302-5-9?v=Oxford+Instruments
    Average 99 stars, based on 1 article reviews
    ca1 excitatory synapses - by Bioz Stars, 2026-08
    99/100 stars
    		  Buy from Supplier
    formation of excitatory synapses  (VANGL2 LTD)
    90
    VANGL2 LTD formation of excitatory synapses
    CLR01 reverts the loss of glutamatergic synapses in cortical neurons caused by pathological Atx3. Rat cortical neurons were transfected with plasmids encoding eGFP, wild‐type eGFP‐Atx3 28Q, or mutant eGFP‐Atx3 84Q. a) Neurons were immunolabeled for MAP2, PSD95, and vGLUT1. <t>Excitatory</t> synapses were detected as PSD95 puncta that colocalized with vGLUT1; scale bar = 10 µm. b) Integrated fluorescence intensity of PSD95 puncta colocalized with vGLUT1 ( n = 33–36 neurons per condition in 3 independent experiments). Statistical analyses were performed using Kruskal–Wallis and Dunn's post‐hoc test (Table , Supporting Information). c) Neurons incubated with 10 µ m CLR03 or CLR01 were immunolabeled for MAP2, PSD95, and VGluT1 clusters; scale bar = 10 µm. d) Integrated fluorescence intensity of PSD95 puncta colocalized with VGluT1 ( n = 35–41 neurons per condition in each of 3 independent experiments. Statistical analyses were performed using Kruskal–Wallis and Dunn's post‐hoc tests (Table , Supporting Information). In panels (c) and (d), boxes show the 25th and 75th percentiles, whiskers range from the minimum to the maximum values, and the horizontal line shows the median value. Mean is represented by “+.” e) GFP‐Atx3 accumulates in the cell body of a fraction of transfected cortical cultures treated with CLR03 or CLR01; scale bars = 300, 15 µm. f) Percentage of cells with GFP‐Atx3 aggregates ( n = 3 independent experiments, 31–35 neurons per condition). Statistical analyses were performed using two‐way ANOVA and Sidak's multiple comparisons post‐hoc tests (Table , Supporting Information). p ‐values are indicated in the graphs shown in panels (b), (d), and (f). Data are represented as mean ± SEM.
    Formation Of Excitatory Synapses, supplied by VANGL2 LTD, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/excitatory+synapses/pm35580729-99-9-4?v=VANGL2+LTD
    Average 90 stars, based on 1 article reviews
    formation of excitatory synapses - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier
    long-term depression of excitatory intracortical synapses  (Rittenhouse Book Distributors)
    90
    Rittenhouse Book Distributors long-term depression of excitatory intracortical synapses
    CLR01 reverts the loss of glutamatergic synapses in cortical neurons caused by pathological Atx3. Rat cortical neurons were transfected with plasmids encoding eGFP, wild‐type eGFP‐Atx3 28Q, or mutant eGFP‐Atx3 84Q. a) Neurons were immunolabeled for MAP2, PSD95, and vGLUT1. <t>Excitatory</t> synapses were detected as PSD95 puncta that colocalized with vGLUT1; scale bar = 10 µm. b) Integrated fluorescence intensity of PSD95 puncta colocalized with vGLUT1 ( n = 33–36 neurons per condition in 3 independent experiments). Statistical analyses were performed using Kruskal–Wallis and Dunn's post‐hoc test (Table , Supporting Information). c) Neurons incubated with 10 µ m CLR03 or CLR01 were immunolabeled for MAP2, PSD95, and VGluT1 clusters; scale bar = 10 µm. d) Integrated fluorescence intensity of PSD95 puncta colocalized with VGluT1 ( n = 35–41 neurons per condition in each of 3 independent experiments. Statistical analyses were performed using Kruskal–Wallis and Dunn's post‐hoc tests (Table , Supporting Information). In panels (c) and (d), boxes show the 25th and 75th percentiles, whiskers range from the minimum to the maximum values, and the horizontal line shows the median value. Mean is represented by “+.” e) GFP‐Atx3 accumulates in the cell body of a fraction of transfected cortical cultures treated with CLR03 or CLR01; scale bars = 300, 15 µm. f) Percentage of cells with GFP‐Atx3 aggregates ( n = 3 independent experiments, 31–35 neurons per condition). Statistical analyses were performed using two‐way ANOVA and Sidak's multiple comparisons post‐hoc tests (Table , Supporting Information). p ‐values are indicated in the graphs shown in panels (b), (d), and (f). Data are represented as mean ± SEM.
    Long Term Depression Of Excitatory Intracortical Synapses, supplied by Rittenhouse Book Distributors, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/excitatory+synapses/pmc08323687-85-18-21?v=Rittenhouse+Book+Distributors
    Average 90 stars, based on 1 article reviews
    long-term depression of excitatory intracortical synapses - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    CLR01 reverts the loss of glutamatergic synapses in cortical neurons caused by pathological Atx3. Rat cortical neurons were transfected with plasmids encoding eGFP, wild‐type eGFP‐Atx3 28Q, or mutant eGFP‐Atx3 84Q. a) Neurons were immunolabeled for MAP2, PSD95, and vGLUT1. Excitatory synapses were detected as PSD95 puncta that colocalized with vGLUT1; scale bar = 10 µm. b) Integrated fluorescence intensity of PSD95 puncta colocalized with vGLUT1 ( n = 33–36 neurons per condition in 3 independent experiments). Statistical analyses were performed using Kruskal–Wallis and Dunn's post‐hoc test (Table , Supporting Information). c) Neurons incubated with 10 µ m CLR03 or CLR01 were immunolabeled for MAP2, PSD95, and VGluT1 clusters; scale bar = 10 µm. d) Integrated fluorescence intensity of PSD95 puncta colocalized with VGluT1 ( n = 35–41 neurons per condition in each of 3 independent experiments. Statistical analyses were performed using Kruskal–Wallis and Dunn's post‐hoc tests (Table , Supporting Information). In panels (c) and (d), boxes show the 25th and 75th percentiles, whiskers range from the minimum to the maximum values, and the horizontal line shows the median value. Mean is represented by “+.” e) GFP‐Atx3 accumulates in the cell body of a fraction of transfected cortical cultures treated with CLR03 or CLR01; scale bars = 300, 15 µm. f) Percentage of cells with GFP‐Atx3 aggregates ( n = 3 independent experiments, 31–35 neurons per condition). Statistical analyses were performed using two‐way ANOVA and Sidak's multiple comparisons post‐hoc tests (Table , Supporting Information). p ‐values are indicated in the graphs shown in panels (b), (d), and (f). Data are represented as mean ± SEM.

    Journal: Advanced Science

    Article Title: Allosteric Modulation of Pathological Ataxin‐3 Aggregation: A Path to Spinocerebellar Ataxia Type‐3 Therapies

    doi: 10.1002/advs.202502216

    Figure Lengend Snippet: CLR01 reverts the loss of glutamatergic synapses in cortical neurons caused by pathological Atx3. Rat cortical neurons were transfected with plasmids encoding eGFP, wild‐type eGFP‐Atx3 28Q, or mutant eGFP‐Atx3 84Q. a) Neurons were immunolabeled for MAP2, PSD95, and vGLUT1. Excitatory synapses were detected as PSD95 puncta that colocalized with vGLUT1; scale bar = 10 µm. b) Integrated fluorescence intensity of PSD95 puncta colocalized with vGLUT1 ( n = 33–36 neurons per condition in 3 independent experiments). Statistical analyses were performed using Kruskal–Wallis and Dunn's post‐hoc test (Table , Supporting Information). c) Neurons incubated with 10 µ m CLR03 or CLR01 were immunolabeled for MAP2, PSD95, and VGluT1 clusters; scale bar = 10 µm. d) Integrated fluorescence intensity of PSD95 puncta colocalized with VGluT1 ( n = 35–41 neurons per condition in each of 3 independent experiments. Statistical analyses were performed using Kruskal–Wallis and Dunn's post‐hoc tests (Table , Supporting Information). In panels (c) and (d), boxes show the 25th and 75th percentiles, whiskers range from the minimum to the maximum values, and the horizontal line shows the median value. Mean is represented by “+.” e) GFP‐Atx3 accumulates in the cell body of a fraction of transfected cortical cultures treated with CLR03 or CLR01; scale bars = 300, 15 µm. f) Percentage of cells with GFP‐Atx3 aggregates ( n = 3 independent experiments, 31–35 neurons per condition). Statistical analyses were performed using two‐way ANOVA and Sidak's multiple comparisons post‐hoc tests (Table , Supporting Information). p ‐values are indicated in the graphs shown in panels (b), (d), and (f). Data are represented as mean ± SEM.

    Article Snippet: To stain for excitatory synapses, anti‐PSD95 antibody (mouse, 1:500, Cell Signaling Technology) and anti‐VGLUT1 antibody (guinea pig, 1:1000, Merck Millipore) were used; dendrites were identified with anti‐MAP2 antibody (chicken, 1:5000, Abcam).

    Techniques: Transfection, Mutagenesis, Immunolabeling, Fluorescence, Incubation